|
KMID : 0380020040190050352
|
|
Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 5 p.352 ~ p.357
|
|
|
Iron Uptake by the Recombinant Yeasts Producing Ferritin Heteropolymers
|
|
Chang Yu-Jung
Park Chung-Woong
Kim Kyung-Suk
|
|
|
Abstract
|
|
|
|
Human ferritin H- and L-chain genes (hfH and hfL) were cloned into the yeast shuttle vector YEp352 containing the GAL1 (galactokinase) and GAL10 (epimerase) divergent promoters and the vectors constructed were used to transform Saccharomyces cerevisiae 2805. SDS-PAGE displayed expression of the introduced hfH and hfL in both recombinant strains of Y1H10L and Y1L10H. The ferritin subunits, that represented ca. and of the soluble proteins in Y1H10L and Y1L10H, were spontaneously assembled into active ferritin heteropolymers. The H subunit content of the purified recombinant human ferritin heteropolymers was proven to reflect the relative expression yield of the subunits. When the cells of 2d culture were incubated with 14.3 mM Fe(2), the cellular iron concentration of Y1H10L and Y1L10H was 1.7 and 2.0 times, respectively, that of the control strain. It is assumed that increase in the iron uptake of the recombinant yeasts is closely related to ferritin expression and H subunit content.
|
|
|
KEYWORD
|
|
|
Iron uptake, yeast, ferritin heteropolymers
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|